Expression of membrane proteins

Production of VLP-s pseudotyped with membrane proteins 

Expression of viral envelope or capsid proteins results in the self-assembly of virus-like particles (VLPs) that provide a great platform for the production of functional membrane proteins with native folding. This kind of pseudotyped VLPs could be used for different approaches, e.g. vaccine development, investigation of receptor functions, screening of antibodies, for immunization and also for purification of different membrane proteins. 

Icosagen produces VLPs pseudotyped with membrane proteins, such as ion-channels, receptors, viral glycoproteins, etc. For the formation of VLPs, we use retroviral (HIV-1 or MLV) and filoviral (ebola) proteins. VLPs could be produced in 293 or CHO-based QMCF cell lines. 




Our advantages

  • Fast and efficient production of VLPs pseudotyped with different receptors, ion-channels, viral glycoproteins, etc.
  • Human-specific posttranslational modification pattern using 293-based cell line


Cell pool generation for membrane protein expression

Icosagen provides services for the fast development of CHO-based cell pools and cell lines, which express membrane proteins. The cell lines could be generated using QMCF Technology in 3 weeks, starting with the cloning of a protein of interest into a QMCF expression vector. 

The cell lines could be used for signal pathway activation assays, for investigation of receptors or ion-channels in batch-clamp assays or ligand binding assay. The cell lines could be also used for membrane protein purification or for development of antibodies against receptors using whole-cell immunization.

The service includes construction of expression vectors, generation of cell population and cell banks, optimization and validation of an assay.




Advantages:

  • Fast and efficient way to generate a stable population of cells expressing a protein of interest; for investigation of signal transduction, screening of active compounds for early stages of drug development
  • Usage of CHO- or U2OS based adherent QMCF cell lines
  • Feasible generation of expression cell banks
  • Assay optimization and validation using Thermo Scientific ArrayScan VTI and appropriate Cellomics software