Proteins

SARS-CoV-2 Trimeric Spike UK VOC 202012/01

P-317-100

Datasheet as
Name SARS-CoV-2 Trimeric Spike UK VOC 202012/01
Description Protein contains amino acids 14-1211 and following mutations: HV 69-70 del, Y144 del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS). Also, two stabilizing prolines (PP) have been added to the S2 domain.
Uniprot ID P0DTC2
Source Human
MW 410 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
Purity >95%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
QC SDS-PAGE, analytical SEC, Octet binding
Shipping Shipped with cold packs
Storage Stored at +4 °C
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€750 / 100µg
€1450 / 500µg
€1950 / 1000µg

Figure 1.  Simply Blue stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike UK VOC 202012/01 . 4-12% gradient gel is used for analysis. Lane 1. 1.3 µg SARS-CoV-2 Trimeric Spike UK VOC 202012/01 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Trimeric Spike UK VOC 202012/01 (+DTT)

Figure 2.  Octet Red96e analysis of SARS-CoV-2 Trimeric Spike UK VOC 202012/01 binding to human ACE2 receptor.