Proteins

SARS-CoV-2 Trimeric Spike Omicron (B.1.1.529)

P-369-100

Datasheet as
Name SARS-CoV-2 Trimeric Spike Omicron (B.1.1.529)
Description Protein contains amino acids 14-1211, A67V, del69-70, T95I, G142D, del143-145, del211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS).
Uniprot ID P0DTC2
Source Human
MW 411,5 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
Bioproperties Measured by its binding ability to ACE2 protein by OCTET RED96 system.
QC SDS-PAGE
NanoDrop A280
Analytical SEC
Octet binding to ACE2
Shipping Shipped on dry ice.
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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