Proteins

SARS-CoV-2 Spike S1 with D614G

P-321-100

Datasheet as
Name SARS-CoV-2 Spike S1 with D614G
Description Protein contains amino acids 14-681, mutation D614G, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
MW 75,82 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity >95%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
Bioproperties Measured by its binding ability to ACE2 protein by OCTET RED96 system.
QC

SDS-PAGE

NanoDrop A280

Shipping Shipped on dry ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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Figure 1.  Coomassie-stained SDS-PAGE analysis of SARS-CoV-2 Spike S1 with D614G. 4-12% gradient gel is used for analysis.

Lane 1. 0.8 µg SARS-CoV-2 Spike S1 with D614G (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Spike S1 with D614G (+DTT)

Figure 2.  Binding analysis of the SARS-CoV-2 Spike S1 with D614G protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.