SARS-CoV-2 Spike S1 UK VOC 202012/01 (alpha)


Datasheet as
Name SARS-CoV-2 Spike S1 UK VOC 202012/01
Description cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1) Protein contains amino acids 14-681, mutations HV 69-70 del, Y144 del, N501Y, A570D, P681H, D614G, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
MW 75,56 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity >95%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
Bioproperties Measured by its binding ability to ACE2 protein by OCTET RED96 system.


NanoDrop A280

Shipping Shipped on dry ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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 Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-COV-2 S1 UK VOC 202012/01 . 4-15% gradient gel is used for analysis. 

Lane 1. 1.3 µg SARS-CoV-2 Spike S1 UK VOC 202012/01 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Spike S1 UK VOC 202012/01 (+DTT)

Figure 2. Binding analysis of the SARS-CoV-2 Spike S1 UK VOC  protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor.  200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.