SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (beta)
P-319-100
Datasheet as| Name | SARS-CoV-2 Spike S1 South Africa VOC 501.V2 |
| Description | Protein contains amino acids 14-681, mutations L18F, D80A, D215G, LAL 242-244 del, R246I, K417N, E484K, N501Y, D614G, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag. |
| MW | 75,45 kDA |
| Host |
CHO-based cell line (expressed by QMCF Technology)
|
| Purification |
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated
through 0.22 µm filter.
|
| Purity |
>95%
|
| Concentration |
1 mg/ml
|
| Buffer |
PBS pH 7.4
|
| Endotoxine |
NA
|
| Bioproperties | Measured by its binding ability to ACE2 protein by OCTET RED96 system. |
| QC |
SDS-PAGE NanoDrop A280 |
| Shipping |
Shipped on dry ice
|
| Storage |
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
|
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€500 / 100µg
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Figure 1.
Coomassie-stained SDS-PAGE analysis of
SARS-CoV-2 Spike S1 South Africa VOC 501.V2. 4-12% gradient gel is used for analysis.
Lane 1. 0.8 µg SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Spike S1 South Africa VOC 501.V2 (+DTT)
Figure 2. Binding analysis of the SARS-CoV-2 Spike S1 South Africa VOC 501.V2. protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.