SARS-CoV-2 Spike S1 Indian B.1.617.1 (kappa)
P-348-100
Datasheet as| Name | SARS-CoV-2 Spike S1 Indian B.1.617.1 (kappa) |
| Description | Protein contains amino acids 14-681, mutations G142D, E154K, L452R, E484Q, D614G, P681R, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag. |
| MW | 79.99 kDa |
| Host |
CHO-based cell line (expressed by QMCF Technology)
|
| Purification |
Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated
through 0.22 µm filter.
|
| Purity | >95% |
| Concentration | 1 mg/ml |
| Buffer |
PBS pH 7.4
|
| Endotoxine | NA |
| Bioproperties |
Measured by its binding ability to ACE2 protein by OCTET RED96 system.
|
| QC | SDS-PAGE NanoDrop A280 |
| Shipping | Shipped on dry ice |
| Storage |
Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
|
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Figure 1. Coomassie-stained SDS-PAGE analysis of
SARS-CoV-2 Spike S1 Indian B.1.617.1. 4-12% gradient gel is used for analysis.
Lane 1. 0.8 µg SARS-CoV-2 Spike S1 Indian B.1.617.1 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Spike S1 Indian B.1.617.1 (+DTT)
Figure 2. Binding analysis of the SARS-CoV-2 Spike S1 Indian B.1.617.1 protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.