Proteins

SARS-CoV-2 Spike S1

P-305-100

Datasheet as
Name SARS-CoV-2 Spike S1
Description cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)  Protein contains amino acids 14-681, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus. 
MW 76,42 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity >90%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
Bioproperties Measured by its binding ability to ACE2 protein by OCTET RED96 system. 
QC

SDS-PAGE

NanoDrop A280

Shipping Shipped on dry-ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-COV-2 S1. 4-15% gradient gel is used for analysis.

Figure 2. Binding analysis of the SARS-CoV-2 Spike S1 protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 25 to 200 nanomols of SARS-CoV-2 Spike S1 protein was bound to the ACE2 receptor.