Proteins

Artemin, human recombinant

P-102-100

Name Artemin, human recombinant
Synonyms Artemin, Enovin, Neublastin
Uniprot ID Q5T4W7
Source Human
MW Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. Protein is homodimer
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified from serum-free CHO growth media by ion-exchange chromatography and gel-filtration. Protein is sterile-filtered through 0.22 µm filter
Concentration 0.43 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
Buffer 10 mM Na-citrate; 150 mM NaCl pH 4.5
Endotoxine Less than 1 EU/mg of protein as determined by LAL method
Bioproperties Product is suitable for cell culture and in vivo testing. Protein is biologically active. Biological activity of purified artemin was tested in in vitro receptor-dependent cellular morphology change induction assay using U2OS cells expressing Ret and GFRα-3 receptor complex
QC SDS-PAGE and Western-Blot analysis
Background Artemin is a ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex. Supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue (www.uniprot.org; Baloh et al. 1998, Neuron; Rosenblad et al. 2000, Eur. J. Biochem)
Shipping Shipped in dry-ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
Related Products Polyclonal and monoclonal antibodies against human Artemin For more information please visit: www.icosagen.com/antibodies
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Figure 1. Coomassie-stained SDS-PAGE and Western Blot analysis on purified recombinant human Artemin. (A) SDS-PAGE analysis in reduced and non-reduced conditions. 2µg of recombinant human Artemin was loaded per lane. Lane 1 Artemin (reduced); Lane 2. Protein size marker (Blue Protein Ladder Prestained, Naxo, 8003);  Lane 3. Artemin (non-reduced);

(B) Western Blot alalysis of recombinant human Artemin.  Anti-human Artemin monoclonal antibody colne 2D12 (Icosagen AS Cat. No 324-100) was used for testing. 2D12 antibody recognizes only native conformation, dimeric (non-reduced) Artemin. 0.2 µg of recombinant Artremin were loaded per lane. Lane 1. Artemin (reduced); Lane 2. Protein size marker Blue (Protein Ladder Prestained, Naxo, 8003); Lane 3. Artemin (non-reduced)