Artemin, human recombinant
P-102-100
Datasheet as| Name | Artemin, human recombinant |
| Synonyms | Artemin, Enovin, Neublastin |
| Uniprot ID | Q5T4W7 |
| Source | Human |
| MW | Approximately 18.8 kDa, a single non-glycosylated polypeptide chain containing 181 amino acids. Protein is homodimer |
| Host | CHO-based cell line (expressed by QMCF Technology) |
| Purification | Purified from serum-free CHO growth media by ion-exchange chromatography and gel-filtration. Protein is sterile-filtered through 0.22 µm filter |
| Concentration | 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard |
| Buffer | 10 mM Na-citrate; 150 mM NaCl pH 4.5 |
| Endotoxine | Less than 1 EU/mg of protein as determined by LAL
method |
| Bioproperties | Product is suitable for cell culture and in vivo testing. Protein is biologically active. Biological activity of purified artemin was tested in in vitro receptor-dependent cellular morphology change induction assay using U2OS cells expressing Ret and GFRα-3 receptor complex |
| QC | SDS-PAGE and Western-Blot analysis |
| Background | Artemin is a ligand for the GFR-alpha-3-RET receptor
complex but can also activate the GFR-alpha-1-RET receptor complex. Supports
the survival of sensory and sympathetic peripheral neurons in culture and also
supports the survival of dopaminergic neurons of the ventral mid-brain. Strong
attractant of gut hematopoietic cells thus promoting the formation Peyer's
patch-like structures, a major component of the gut-associated lymphoid tissue (www.uniprot.org;
Baloh et al. 1998, Neuron; Rosenblad et al. 2000, Eur. J. Biochem) |
| Shipping | Shipped in dry-ice |
| Storage | Store at -70°C upon receipt. Recommended to aliquot
into smaller quantities. Avoid repeated freeze-thaw cycles |
| Related Products | Polyclonal and monoclonal antibodies against human Artemin
For more information please visit: www.icosagen.com/antibodies |
A B
Figure 1. Coomassie-stained SDS-PAGE and Western Blot analysis on purified recombinant human Artemin. (A) SDS-PAGE analysis in reduced and non-reduced conditions. 2µg of recombinant human Artemin was loaded per lane. Lane 1 Artemin (reduced); Lane 2. Protein size marker (Blue Protein Ladder Prestained, Naxo, 8003); Lane 3. Artemin (non-reduced);
(B) Western Blot alalysis of recombinant human Artemin. Anti-human Artemin monoclonal antibody colne 2D12 (Icosagen AS Cat. No 324-100) was used for testing. 2D12 antibody recognizes only native conformation, dimeric (non-reduced) Artemin. 0.2 µg of recombinant Artremin were loaded per lane. Lane 1. Artemin (reduced); Lane 2. Protein size marker Blue (Protein Ladder Prestained, Naxo, 8003); Lane 3. Artemin (non-reduced)