Datasheet as
Name SARS-CoV-2 S1 RBD
Source cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)

Protein contains amino acids 331-524, two extra amino acids (AS) in N-terminus and His-6 tag at C-terminus.
MW 23 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity >95%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
Bioproperties Measured by its binding ability to ACE2 protein by OCTET RED96 system.

NanoDrop A280
Shipping Shipped on dry-ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-COV-2 S1 RBD. 4-15% gradient gel is used for analysis

Figure 2. Binding analysis of the SARS-CoV-2 S1 RBD protein to hACE2 receptor. hACE2 -Fc protein was bound to Protein G sensor. 25 to 200 nanomols of SARS-CoV-2 S1 RBD protein was bound to the ACE2 receptor.