NGF, human recombinant
P-104-100
Datasheet as| Name | NGF, human recombinant |
| Synonyms | Beta-nerve growth factor, NGFB |
| Uniprot ID | P01138 |
| Source | Human |
| MW | Mature NGF, Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids |
| Host | CHO-based cell line (expressed by QMCF Technology) |
| Purification | Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media. Protein is sterile-filtrated through 0.22 µm filter |
| Concentration | 1 mg/ml |
| Buffer | PBS pH 7.4 |
| Endotoxine | Less than 1EU/mg of protein as determined by LAL method |
| QC | Coomassie-stained SDS-PAGE (Figure 1) |
| Background | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (UniProt) |
| Shipping | Shipped on dry ice |
| Storage | Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles |
| Related Products | Monoclonal antibodies against human NGF. For more information please visit: www.icosagen.com/antibodies, human neural growth factors. For more information about protein production please visit: www.icosagen.ee/services/protein-production |
Figure 1. Coomassie-stained SDS-PAGE analysis of human NGF. Lane 1. Protein size marker (Blue Protein Ladder Prestained, Naxo 8003). Lane 2. 2 μg of NGF (non-reduced);