RET-His, human recombinant
P-124-100
Datasheet as| Name | RET-His, human recombinant |
| Description | Protein contains only extracellular domain of the RET (aa 29-635) |
| Synonyms | Cadherin family member 12, Proto-oncogene c-Ret |
| Uniprot ID | P07949 |
| Source | Human |
| MW | Approximately 68.6 kDa, a single non-glycosylated polypeptide chain containing 613 amino acids including C-terminal hexahistidine tag |
| Host | CHO-based cell line (expressed by QMCF Technology) |
| Purification | Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media |
| Concentration | 0.23 mg/ml |
| Buffer | PBS pH 7.4 |
| Endotoxine | Not tested |
| QC | Coomassie-stained SDS-PAGE 12%, 2 µg per lane |
| Background | Receptor
tyrosine-protein kinase involved in numerous cellular mechanisms including cell
proliferation, neuronal navigation, cell migration, and cell differentiation
upon binding with glial cell derived neurotrophic factor family ligands.
Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and
positional information. Required for the molecular mechanisms orchestration
during intestine organogenesis; involved in the development of enteric nervous
system and renal organogenesis during embryonic life, and promotes the
formation of Peyer's patch-like structures, a major component of the
gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by
caspase in sympathetic neurons and mediates cell migration in an integrin (e.g.
ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural
crest. Active in the absence of ligand, triggering apoptosis through a
mechanism that requires receptor intracellular caspase cleavage. Acts as a
dependence receptor; in the presence of the ligand GDNF in somatotrophs (within
pituitary), promotes survival and down regulates growth hormone (GH)
production, but triggers apoptosis in absence of GDNF. Regulates nociceptor
survival and size. Triggers the differentiation of rapidly adapting (RA)
mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers;
these diseases are characterized by aberrant integrins-regulated cell migration |
| Shipping | Shipped on dry ice |
| Storage | Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles |
Figure 1. SDS-PAGE analysis of human RET635 visualized by PageBlue protein staining solution (Fermentas). Lane 1. 2 µg of human RET635 (reduced); Lane 2. Protein size marker ( Prestained protein ladder, 8003, Naxo); Lane 3. 2 µg of human RET635 (non-reduced)