DNase I, human recombinant
P-200-100
Datasheet as| Name | DNase I, human recombinant |
| Synonyms | Deoxyribonuclease I |
| Uniprot ID | P24855 |
| Source | Human |
| MW | Approximately 29.2 kDa, a single non-glycosylated polypeptide chain containing 260 amino acids |
| Host | CHO-based cell line (expressed by QMCF Technology) |
| Purification | Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media |
| Concentration | 0.5 mg/ml |
| Buffer | PBS pH 7.4 |
| Bioproperties | Protein is biologically active |
| QC | Coomassie-stained SDS-PAGE 12%, 2 µg per lane |
| Background | Endonucleolytic
cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products.
Cofactor: Ca2+, Mg2+ |
| Shipping | Shipped on dry ice |
| Storage | Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles |
| Related Products | Monoclonal antibodies against human DNaseI. For more information please visit:www.icosagen.com/antibodies. |
Figure 1. SDS-PAGE analysis of human DNase I visualized by PageBlue protein staining solution (Fermentas). Lane 1. 2 µg of hDNaseI (non-reduced); Lane 2. Protein size marker ( Prestained protein ladder, 8003, Naxo); Lane 3. 2 µg of hDNaseI (reduced)