Proteins

DNase I, human recombinant

P-200-100

Name DNase I, human recombinant
Synonyms Deoxyribonuclease I
Uniprot ID P24855
Source Human
MW Approximately 29.2 kDa, a single non-glycosylated polypeptide chain containing 260 amino acids
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media
Concentration 0.5 mg/ml
Buffer PBS pH 7.4
Bioproperties Protein is biologically active
QC Coomassie-stained SDS-PAGE 12%, 2 µg per lane
Background Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products. Cofactor:  Ca2+, Mg2+
Shipping Shipped on dry ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
Related Products Monoclonal antibodies against human DNaseI. For more information please visit:www.icosagen.com/antibodies.
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€231 / 10µg
€473 / 50µg
€840 / 100µg
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Figure 1. SDS-PAGE analysis of human DNase I visualized by PageBlue protein staining solution (Fermentas). Lane 1. 2 µg of hDNaseI (non-reduced); Lane 2. Protein size marker ( Prestained protein ladder, 8003, Naxo); Lane 3. 2 µg of hDNaseI (reduced)