DNase I, bovine recombinant


Datasheet as
Name DNase I, bovine recombinant
Synonyms Deoxyribonuclease I
Uniprot ID P00639
Source Bovine
MW Approximately 29.1 kDa, a single non-glycosylated polypeptide chain containing 260 amino acids
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media
Concentration 0.1 mg/ml
Buffer 10mM Ca-acetate pH 5.0, 140 mM NaCl, 5mM MgCl2
Bioproperties Protein is biologically active
QC Coomassie-stained SDS-PAGE 12%, 2 µg per lane
Background Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products. Cofactor:  Ca2+, Mg2+
Shipping Shipped on dry ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
Related Products Monoclonal antibodies against bovine DNaseI. For more information please
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€249 / 10µg
€511 / 50µg
€908 / 100µg

Figure 1. SDS-PAGE analysis of bovine DNase I visualized by PageBlue protein staining solution (Fermentas). Lane 1. 2 µg of bDNaseI (reduced); Lane 2. Protein size marker ( Prestained protein ladder, 8003, Naxo); Lane 3. 2 µg of bDNaseI (non-reduced)