BDNF, human recombinant
P-105-100
Datasheet as| Name | BDNF, human recombinant |
| Synonyms | Brain-derived neurotrophic factor, Abrineurin |
| Uniprot ID | P23560 |
| Source | Human |
| MW | Mature BDNF, Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 118 amino acids |
| Host | CHO-based cell line (expressed by QMCF Technology) |
| Purification | Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media. Protein is sterile-filtrated through 0.22 µm filter |
| Concentration | 1 mg/ml |
| Buffer | PBS pH 7.4 |
| Endotoxine | Less than 1EU/mg of protein as determined by LAL method |
| QC | Coomassie-stained SDS-PAGE analysis (Figure 1) |
| Background | BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (UniProt) |
| Shipping | Shipped on dry ice |
| Storage | Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles |
| Related Products | Monoclonal antibodies against human BDNF. For more information please visit: www.icosagen.com/antibodies, human neural growth factors. For more information about protein production please visit: www.icosagen.ee/services/protein-production |
Figure 1. Coomassie-stained SDS-PAGE analysis of human BDNF. Lane 1. 2 µg of BDNF (non-reduced); Lane 2. Protein size marker (Blue Protein Ladder Prestained, Naxo 8003) Lane 3. 2 µg of BDNF (reduced).