Proteins

2B4-His, human recombinant

P-404-100

Name 2B4
Description Protein contains only extracellular domain of the human 2B4 (aa 22-229)
Synonyms NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, SLAM family member 4, Signaling lymphocytic activation molecule 4, CD_antigen: CD244
Uniprot ID Q9BZW8
Source Human 
MW Approximately 24.1 kDa, a single non-glycosylated polypeptide chain containing 214 amino acids including C-terminal hexahistidine tag
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified by Ni-affinity chromatography, ion-exchange chromatography and desalting chromatography from serum-free CHO growth media
Concentration 1.1 mg/ml
Buffer PBS pH 6.0
Endotoxine Not tested
QC Coomassie-stained SDS-PAGE 15%, 2 µg per lane
Background Modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family
Shipping Shipped on dry ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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Figure 1. SDS-PAGE analysis of human 2B4 visualized by PageBlue protein staining solution (Fermentas). Lane 1. 2 µg of human 2B4 (reduced); Lane 2. Protein size marker ( Prestained protein ladder, 8003, Naxo); Lane 3. 2 µg of human 2B4 (non-reduced)