Proteins

MANF, human recombinant

P-101-100

Name MANF, human recombinant
Synonyms Mesencephalic astrocyte-derived neurotrophic factor – MANF; ARMET; ARP
Uniprot ID P55145
Source Human
MW Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media
Concentration 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard
Buffer PBS pH 7.4, concentration
Endotoxine Less than 1EU/mg of protein as determined by LAL method
Bioproperties Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat 6-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke
QC SDS-PAGE and Western-Blot analysis (Figure 1 and 2); Mass-spectroscopy: Purified human MANF is characterized as homogenous material by mass-spectroscopy
Background MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
Shipping Shipped in dry-ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
Related Products Polyclonal and monoclonal antibodies against human MANF For more information please visit: www.icosagen.com/antibodies
References Lindahl, M et al. 2014. MANF is Indispensable for the Proliferation and Survival of Pancreatic β Cells. Cell Reports 7, 366-375
Bäck, S et al. 2013. Gene therapy with AAV2-CDNF provides functional benefits in a rat model of Parkinson's disease.Brain and Behavior 3(2): 75-88
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Figure 1. SDS-PAGE analysis of human MANF visualized by PageBlue protein staining solution (Fermentas). Line 1. Protein size marker (PageRuler Prestained protein ladder, Fermentas); Line 2. 10 µg of MANF.

Figure 2. Western Blot testing of human MANF using anti-MANF polyclonal antibody (Icosagen AS Cat. No 310-100). Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Recombinant MANF expressed into the supernatant of CHO cell culture medium