Recombinant monoclonal antibody to human MANF
342-100
Datasheet as| Name | Recombinant monoclonal antibody to human MANF |
| Immunogen | Human MANF |
| Immunogen Description | Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant |
| Uniprot ID | P55145 |
| Alternative Names | ARMET, ARP |
| Clonality | Recombinant monoclonal antibody |
| Clone | 3D4, chicken-mouse IgG2a chimeric antibody |
| Class | mouse IgG2a |
| Reactivity | Human MANF |
| Application | ELISA, IF, IHC-C |
| Protocol | ELISA 0.02 to 0.1 µg/ml; IF 1 to 20 µg/ml; IHC-C 5 µg/ml. Conformational antibody, not suitable for Western Blot application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | MabSelect affinity chromatography |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Monoclonal and polyclonal antibodies and ELISA kit to human MANF. For more information visit www.icosagen.com/antibodies or http://icosagen.com/products/elisa-kits |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta |
| References | - |
| Limitations | This product is for research use only |
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Price per quantity:
€308 / 100µg
€1231 / 500µg
€1999 / 1000µg
Figure 1. IF testing of anti-MANF monoclonal antibody (342-100). Immunofluorescence detection of human MANF expressed in human U2OS cells by chimeric monoclonal antibody to hMANF (342-100). Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. Upper panel: MANF-expressing U2OS cells. Lower panel: Negative control (non-transfected cells)
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Figure 2: Immunohistochemical analysis of MANF monoclonal antibody (342-100). Analysis was performed using mouse pancreas cryosections. Antibody concentration of 5 µg/ml was used. A. WT mouse pancreas. B. MANF KO mouse pancreas