Antibodies

Recombinant monoclonal antibody to human MANF

342-100

Datasheet as
Name Recombinant monoclonal antibody to human MANF
Immunogen Human MANF
Immunogen Description Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Uniprot ID P55145
Alternative Names ARMET, ARP
Clonality Recombinant monoclonal antibody
Clone 3D4, chicken-mouse IgG2a chimeric antibody
Class mouse IgG2a
Reactivity Human MANF
Application ELISA, IF, IHC-C
Protocol

ELISA 0.02 to 0.1 µg/ml; IF 1 to 20 µg/ml; IHC-C 5 µg/ml. 

Conformational antibody, not suitable for Western Blot application. 

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification MabSelect affinity chromatography
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Monoclonal and polyclonal antibodies and ELISA kit to human MANF.  For more information visit www.icosagen.com/antibodies or http://icosagen.com/products/elisa-kits
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
References -
Limitations This product is for research use only
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€299 / 100µg

Figure 1. IF testing of anti-MANF monoclonal antibody (342-100). Immunofluorescence detection of human MANF expressed in human U2OS cells by chimeric monoclonal  antibody to hMANF (342-100). Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. Upper panel: MANF-expressing U2OS cells. Lower panel: Negative control (non-transfected cells)
A

B

Figure 2: Immunohistochemical analysis of MANF monoclonal antibody (342-100). Analysis was performed using mouse pancreas cryosections. Antibody concentration of 5 µg/ml was used. A. WT mouse pancreas. B. MANF KO mouse pancreas