Recombinant monoclonal antibody to human MANF
342-100
Datasheet asName | Recombinant monoclonal antibody to human MANF |
Immunogen | Human MANF |
Immunogen Description | Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant |
Uniprot ID | P55145 |
Alternative Names | ARMET, ARP |
Clonality | Recombinant monoclonal antibody |
Clone | 3D4, chicken-mouse IgG2a chimeric antibody |
Class | mouse IgG2a |
Reactivity | Human MANF |
Application | ELISA, IF, IHC-C |
Protocol | ELISA 0.02 to 0.1 µg/ml; IF 1 to 20 µg/ml; IHC-C 5 µg/ml. Conformational antibody, not suitable for Western Blot application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
Purification | MabSelect affinity chromatography |
Buffer | PBS pH 7.4, with 0.1% sodium azide |
Unit Size | 100 µg |
Related Products | Monoclonal and polyclonal antibodies and ELISA kit to human MANF. For more information visit www.icosagen.com/antibodies or http://icosagen.com/products/elisa-kits |
Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
Storage | Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
Background | MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta |
References | - |
Limitations | This product is for research use only |
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€323 / 100µg
€1292 / 500µg
€2098 / 1000µg
Figure 1. IF testing of anti-MANF monoclonal antibody (342-100). Immunofluorescence detection of human MANF expressed in human U2OS cells by chimeric monoclonal antibody to hMANF (342-100). Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. Upper panel: MANF-expressing U2OS cells. Lower panel: Negative control (non-transfected cells)
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Figure 2: Immunohistochemical analysis of MANF monoclonal antibody (342-100). Analysis was performed using mouse pancreas cryosections. Antibody concentration of 5 µg/ml was used. A. WT mouse pancreas. B. MANF KO mouse pancreas