Antibodies

Rabbit polyclonal antibody to human ribonuclease 8

430-100

Datasheet as
Name Rabbit polyclonal antibody to human Ribonuclease 8 (RNase 8)
Immunogen Human Ribonuclease 8
Immunogen Description Recombinant human RNase 8 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Uniprot ID Q8TDE3
Alternative Names RNase 8
Clonality Rabbit polyclonal
Class IgG
Reactivity human RNase 8, cross-reactivity to human RNase 7
Application ELISA, WB, IF
Protocol

ELISA 1 to 2 ng/ml; WB 0,1 to 0,5 µg/ml; IF 1 to 10 µg/ml

Antibody optimal dilution has to be established practically for each  antigen and assay format
Purification RNase 8 - affinity purified
Buffer Concentrated ammonium sulphate in PBS pH 7.4
Unit Size 100 µg
Related Products Monoclonal and polyclonal antibodies to human RNase 8.  For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background The RNase 8 gene is linked to seven other RNase A superfamily genes on chromosome 14. It is expressed prominently in the placenta, but is not detected in any other tissues examined. The ribonucleolytic activity of recombinant human RNase 8 is among the lowest of members of this superfamily and it exhibits neither antiviral nor antibacterial activities characteristic of some other RNase A ribonucleases (reviewd from Zhang et al. 2002, NAR, 30 (5) 1169-1175).
References -
Limitations This product is for research use only
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€299 / 100µg













Figure 1.
 Western Blot analysis of polyclonal RNase 8 antibody. Human RNase 8 was expressed by CHOEBNALT85 cell line. 50 ng of purified protein was loaded per line. Line 1. 50 ng non-reduced RNase 8; Line 2. Prestained Protein Ladder, Naxo 8003. Primary antibody concentration 1 µg/ml was used for analysis.


Figure 2. Immunofluorescence detection of human RNase 8 expressed in U2OS cells by anti-RNase 8 polyclonal antibody. Anti-RNase 8 antibody  concentration in IF experiment was 1 µg/ml. Goat ant-rabbit AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for RNase 8 specific signal and blue for nuclei. A. Human RNase 8 - expressing U2OS cells; B. Negative control (non-transfected U2OS cells).