Mouse monoclonal antibody to Human Cardiac Troponin I
103-100
Datasheet as| Name |
Mouse monoclonal antibody to Human Cardiac Troponin I
|
| Immunogen | Human cardiac troponin I |
| Immunogen Description |
Native, affinity purified human cardiac troponin I
|
| Uniprot ID |
P19429
|
| Clonality |
Mouse monoclonal
|
| Clone | 1D12 |
| Class | IgG1 |
| Reactivity |
human cardiac troponin I
|
| Dissociation constant (KD) |
4.3 x 10-10 M
|
| Application |
ELISA, WB |
| Protocol |
ELISA, 0.05-0.1 μg/ml; WB, 0.2 to
0.5
μg/ml
|
| Purification |
Protein G purification
|
| Buffer |
PBS pH 7.4; 0.1% NaN3
|
| Unit Size |
100 µg
|
| Related Products | Monoclonal antibodies to human cardiac troponin I. For more information visit www.icosagen.com/antibodies
|
| Shipping |
This product is shipped in non-frozen liquid form in ambient conditions
|
| Storage |
Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
|
| Background |
Troponin I is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it, myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle (Wikipedia)
|
| Limitations |
This product is for research use only
|
Buy now
€299 / 100µg
Figure 1. Western Blot testing of mouse monoclonal antibody (103-100) to human cardiac troponin I. Lane 1. Recombinant Cardiac Troponin I, 5ng is loaded per lane. Lane 2. Recombinant Cardiac Troponin I, 10ng is loaded per lane. Lane 3. Recombinant Cardiac Troponin I, 50ng is loaded per lane. Antibody concentration 0.2 µg/ml Lane 4. PageRuler Prestained Protein Ladder (Naxo 8004).