Mouse monoclonal antibody to human BDNF
|Name||Mouse monoclonal antibody to human BDNF|
|Immunogen Description||Recombinant human BDNF protein purified from E. coli|
|Reactivity||Human, mouse, rat, guinea pig|
|Application||ELISA, WB, IF
|Protocol||ELISA 0.1 to 0.2 µg/ml; WB 0.2 to 2 µg/ml; IF 0.33 to 20 µg/ml|
|Purification||Protein G purification|
|Buffer||PBS pH 7.4 with 0.1% sodium azide|
|Unit Size||100 µg|
|Shipping||This product is shipped in non-frozen liquid form in ambient conditions|
|Storage||Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles|
|Background||Brain-derived neurotrophic factor (BDNF) plays an important role in activity-dependent synaptic plasticity such as long-term potentiation. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses|
|Limitations||This product is for research use only
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€299 / 100µg
Figure 1. Western Blot analysis of anti-BDNF monoclonal antibody 3B2. Lane 1: 10 µl of CHO cell culture supernatant containing BDNF was loaded into the gel under reducing conditions. Antibody concentration 5 µg/ml. HRP-conjugated Goat anti-Mouse IgG was used as secondary antibody. Lane 2: Protein size marker
Figure 2. Western Blot testing of anti-BDNF monoclonal antibody 3B2. Antibody concentrations of 1 µg/ml was used. 2
Lanes 1 recombiant BDNF 1 µg , Lines 2 and 3 – neuron lysates. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.
Figure 3. Immunofluorescence detection of hBDNF expression in U2OS cells by anti-BDNF monoclonal antibody 3B2. Antibody concentration 0.33 µg/ml. Goat anti-mouse AlexaFluor488 was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for BDNF specific signal and blue for nuclei. A. proBDNF-expressing U2OS cells; B. Negative control (non-transfected U2OS cells).
Figure 4. Immunohistochemistry testing of anti-BDNF monoclonal antibody 3B2. Analysis was performed using FFPE human cerebral cortex tissue sections from Alzheimer's disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAKO EnVisionTM Detection System, Peroxidase/DAB was used for visualization. Sections were counterstained with toluidine blue and mounted with Eukitt mounting medium.
A. BDNF staining by monoclonal antibody 3B2; B.Negative staining without primary antibody