Mouse monoclonal antibody to human BDNF
327-100
Datasheet as| Name | Mouse monoclonal antibody to human BDNF |
| Immunogen | Human BDNF |
| Immunogen Description | Recombinant mature human BDNF protein produced in E. coli |
| Uniprot ID | P23560 |
| Alternative Names | Abrineurin |
| Clonality | Mouse monoclonal |
| Clone | 3C11 |
| Class | IgG1 |
| Reactivity | human, mouse, rat, guinea pig |
| Application | ELISA, WB, IF |
| Protocol | ELISA
0.02 to 1 µg/ml; WB
0.1 to 1 µg/ml under reducing conditions; IF 2 to 20 µg/ml. Monoclonal antibody working amount has to be established practically for each particular antigen and assay format. |
| Purification | Protein G purified |
| Buffer | PBS pH 7.4, with 0.1% sodium azide. |
| Unit Size | 100 µg |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles. |
| Background | Brain-derived neurotrophic factor (BDNF) plays an important role in activity-dependent synaptic plasticity such as long-term potentiation. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses |
| References | Zunino, G. Messina, A. Sgadò, P. Baj, G. Casarosa, S. Bozzi, Y. Brain-derived neurotrophic factor signaling is altered in the forebrain of Engrailed-2 knockout mice. Neuroscience. 2016 Mar 14 |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€323 / 100µg
€1292 / 500µg
€2098 / 1000µg
€1292 / 500µg
€2098 / 1000µg
Figure 1. Western Blot testing of anti-BDNF monoclonal antibody 3C11 (5 µg/ml). Different amounts (1, 10 and 100 ng) of recombinant hBDNF (E. coli produced) was loaded per lane. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.
Figure 2. Western Blot testing of anti-BDNF monoclonal antibody 3C11 (5 µg/ml). HRP-conjugated goat anti-mouse antibody was used as secondary antibody. 10 µl of CHO supernatant containing BDNF was loaded per lane.
Figure 3. Western Blot testing of anti-BDNF monoclonal antibody 3C11. Antibody concentrations of 1 µg/ml and 0.2 µg/ml was used. 20 µg of cell lysate was loaded per lane. Lanes 1 and 2 – rat astrocytes’ culture preparation. Lanes 3 and 4 – rat hippocampal neuron culture lysate. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.