Antibodies

Mouse monoclonal antibody to human BDNF

327-100

Datasheet as
Name Mouse monoclonal antibody to human BDNF
Immunogen Human BDNF
Immunogen Description Recombinant mature human BDNF protein produced in E. coli
Uniprot ID P23560
Alternative Names Abrineurin
Clonality Mouse monoclonal
Clone 3C11
Class IgG1
Reactivity human, mouse, rat, guinea pig
Application ELISA, WB, IF
Protocol ELISA 0.2 to 1 µg/ml; WB 0.1 to 1 µg/ml under reducing conditions; IF 2 to 20 µg/ml.
Monoclonal antibody working amount has to be established practically for each particular antigen and assay format.
Purification Protein G purified
Buffer PBS pH 7.4, with 0.1% sodium azide.
Unit Size 100 µg
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Background Brain-derived neurotrophic factor (BDNF) plays an important role in activity-dependent synaptic plasticity such as long-term potentiation. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses
References Zunino, G. Messina, A. Sgadò, P. Baj, G. Casarosa, S. Bozzi, Y. Brain-derived neurotrophic factor signaling is altered in the forebrain of Engrailed-2 knockout mice.
Neuroscience. 2016 Mar 14
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. Western Blot testing of anti-BDNF monoclonal antibody 3C11 (5 µg/ml). Different amounts (1, 10 and 100 ng) of  recombinant hBDNF (E. coli produced) was loaded per lane. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.














Figure 2. Western Blot testing of anti-BDNF monoclonal antibody 3C11 (5 µg/ml). HRP-conjugated goat anti-mouse antibody was used as secondary antibody. 10 µl of CHO supernatant containing BDNF was loaded per lane.



Figure 3. Western Blot testing of anti-BDNF monoclonal antibody 3C11. Antibody concentrations of 1 µg/ml and 0.2 µg/ml was used. 20 µg of cell lysate was loaded per lane. Lanes 1 and 2 – rat astrocytes’ culture preparation. Lanes 3 and 4 – rat hippocampal neuron culture lysate. Photo courtesy of Indrek Koppel and Tõnis Timmusk, Tallinn Technical University, Institute of Gene Technology.