Mouse monoclonal antibody to human Ribonuclease 7
408-100
Datasheet as| Name | Mouse
monoclonal antibody to human Ribonuclease 7 |
| Immunogen | Human Ribonuclease 7 |
| Immunogen Description | Recombinant human RNase 7 protein produced by
CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line |
| Uniprot ID | Q9H1E1 |
| Alternative Names | RNase 7, SAP-2 (Skin-derived antimicrobial
protein 2) |
| Clonality | Mouse monoclonal |
| Clone | 4F9 |
| Class | IgG1 |
| Reactivity | Human Ribonuclease
7 |
| Application | ELISA, WB in non-reduced conditions, IHC, IF |
| Protocol | ELISA 0.02 to 0.05 µg/ml; WB 0.5 to 1 µg/ml; IF 1 to 10 µg/ml; IHC 5 to 20 µg/ml Monoclonal antibody optimal dilution has to be established practically for each antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1 % sodium azide |
| Unit Size | 100 µg |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at -20 °C to -70 °C upon receipt. Divide antibody
into aliquots prior usage. Avoid multiple freeze-thaw cycles as product
degradation may result |
| Background | RNase 7 exhibit potent ribonuclease activity and thus
may contribute to the well-known ribonuclease activity of human skin. RNase 7
revealed broad spectrum antimicrobial activity against many pathogenic
microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder,
2002) |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
Figure 1. Western Blot analysis of RNase 7 antibody 4F9. Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture supernatant was loaded per line. Line 1. Prestained Protein Ladder, Naxo 8003; Line 2. non-reduced conditions; Line 3. Reduced conditions
Figure
2. Immunohistochemistry analysis of of RNase 7
antibody 4F9. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 4F9
1:500 was used as primary antibody. Biotinylated anti-mouse antibody was used
as secondary antibody. Streptavidin-HRP conjugate was used for visualization