Antibodies

Mouse monoclonal antibody to human Ribonuclease 7

408-100

Datasheet as
Name Mouse monoclonal antibody to human Ribonuclease 7
Immunogen Human Ribonuclease 7
Immunogen Description Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Uniprot ID Q9H1E1
Alternative Names RNase 7, SAP-2 (Skin-derived antimicrobial protein 2)
Clonality Mouse monoclonal
Clone 4F9
Class IgG1
Reactivity Human Ribonuclease 7
Application

ELISA, WB in non-reduced conditions, IHC, IF

Protocol ELISA 0.02 to 0.05 µg/ml; WB 0.5 to 1 µg/ml; IF 1 to 10 µg/ml; IHC 5 to 20 µg/ml
Monoclonal antibody optimal dilution has to be established practically for each antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1 % sodium azide
Unit Size 100 µg
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20 °C to -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background RNase 7 exhibit potent ribonuclease activity and thus may contribute to the well-known ribonuclease activity of human skin. RNase 7 revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder, 2002)
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. Western Blot analysis of RNase 7 antibody 4F9. Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture supernatant was loaded per line. Line 1. Prestained Protein Ladder, Naxo 8003; Line 2. non-reduced conditions; Line 3. Reduced conditions 


Figure 2. Immunohistochemistry analysis of of RNase 7 antibody 4F9. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 4F9 1:500 was used as primary antibody. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization