Mouse monoclonal antibody to human Ribonuclease 7
407-100
Datasheet as| Name | Mouse
monoclonal antibody to human Ribonuclease 7 |
| Immunogen | Human Ribonuclease 7 |
| Immunogen Description | Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line |
| Uniprot ID | Q9H1E1 |
| Alternative Names | RNase 7, SAP-2 (Skin-derived antimicrobial
protein 2) |
| Clonality | Mouse monoclonal |
| Clone | 4C4 |
| Class | IgG1 |
| Reactivity | Human Ribonuclease
7 |
| Application | ELISA, WB in non-reduced conditions; IHC,IF |
| Protocol | ELISA 0.05 to 0.1 µg/ml; WB 0.5 to 1 µg/ml; IF 0.2 to 20 µg/ml; IHC 2 to 20 µg/ml; Monoclonal antibody optimal dilution has to be established practically for each antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Mono- and polyclonal antibodies to nucleases. For more details visit http://www.icosagen.com/antibodies. Human RNase 7 ELISA kit. For more details visit http://icosagen.edicy.co/products/elisa-kits |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at -20 or -70 °C upon receipt. Divide antibody
into aliquots prior usage. Avoid multiple freeze-thaw cycles as product
degradation may result |
| Background | RNase 7 exhibit potent ribonuclease activity and thus
may contribute to the well-known ribonuclease activity of human skin. RNase 7
revealed broad spectrum antimicrobial activity against many pathogenic
microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder,
2002) |
| References | - |
| Limitations | This
product is for research use only |
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€299 / 100µg
Figure
1. Western Blot analysis of RNase 7 antibody 4C4.
Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture
supernatant was loaded per lane. Lane 1 non-reduced conditions; Lane 2 Reduced conditions; Lane 3 Prestained Protein Ladder, Naxo 8003
A. B.
Figure 2. Immunofluorescence testing anti-RNase7 monoclonal antibody 4C4. Assay was carried out with U2OS cells expressing RNase 7. Antibody concentration of 0.5 ug/ml was used. Nuclei were counterstained by DAPI. A. U2OS cells expressing RNase 7; B. Negative control (non-transfected U2OS cells)
Figure
3. Immunohistochemistry analysis of RNase 7
antibody 4C4. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 4C4 (dilution 1:500) was used as primary antibody. Biotinylated anti-mouse antibody was used
as secondary antibody. Streptavidin-HRP conjugate was used for visualization