Antibodies

Mouse monoclonal antibody to human Ribonuclease 7

407-100

Datasheet as
Name Mouse monoclonal antibody to human Ribonuclease 7
Immunogen Human Ribonuclease 7
Immunogen Description Recombinant human RNase 7 protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Uniprot ID Q9H1E1
Alternative Names RNase 7, SAP-2 (Skin-derived antimicrobial protein 2)
Clonality Mouse monoclonal
Clone 4C4
Class IgG1
Reactivity Human Ribonuclease 7
Application

ELISA, WB in non-reduced conditions; IHC,IF

Protocol ELISA 0.05 to 0.1 µg/ml; WB 0.5 to 1 µg/ml; IF 0.2 to 20 µg/ml; IHC 2 to 20 µg/ml; Monoclonal antibody optimal dilution has to be established practically for each antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Mono- and polyclonal antibodies to nucleases. For more details visit http://www.icosagen.com/antibodies
Human RNase 7 ELISA kit. For more details visit http://icosagen.edicy.co/products/elisa-kits
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20 or -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result
Background RNase 7 exhibit potent ribonuclease activity and thus may contribute to the well-known ribonuclease activity of human skin. RNase 7 revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity (lethal dose of 90% < 30 nm) against a vancomycin-resistant Enterococcus faecium (Harder and Schröder, 2002)
References -
Limitations This product is for research use only
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€299 / 100µg



Figure 1. Western Blot analysis of RNase 7 antibody 4C4. Human RNase 7 was expressed by CHOEBNALT85 cell line. 10 µl of cell culture supernatant was loaded per lane. Lane 1 non-reduced conditions; Lane 2 Reduced conditions; Lane 3 Prestained Protein Ladder, Naxo 8003

A.                         B.

Figure 2. Immunofluorescence testing anti-RNase7 monoclonal antibody 4C4. Assay was carried out with U2OS cells expressing RNase 7. Antibody concentration of 0.5 ug/ml was used. Nuclei were counterstained by DAPI. A. U2OS cells expressing RNase 7; B. Negative control (non-transfected U2OS cells)


Figure 3. Immunohistochemistry analysis of RNase 7 antibody 4C4. Analysis was performed using paraffin-embedded human skin tissue sample. Anti-RNase 7 antibody 4C4 (dilution 1:500) was used as primary antibody. Biotinylated anti-mouse antibody was used as secondary antibody. Streptavidin-HRP conjugate was used for visualization