Mouse monoclonal antibody to human Artemin
326-100
Datasheet as| Name | Mouse
monoclonal antibody to human Artemin |
| Immunogen | Human Artemin |
| Immunogen Description | Recombinant human Artemin protein produced using
CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Immunogen is purified from cell culture supernatant |
| Uniprot ID | Q5T4W7 |
| Alternative Names | Enovin, Neublastin |
| Clonality | Mouse monoclonal |
| Clone | 2G7 |
| Class | IgG1 |
| Reactivity | Human Artemin |
| Application | ELISA, IF, IHC |
| Protocol | ELISA
0.2 to 1 µg/ml; IF 1 to 20 µg/ml; IHC 5 µg/ml. |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Monoclonal and polyclonal antibodies and ELISA kit to human Artemin. For more information visit www.icosagen.com/antibodies or http://icosagen.com/products/elisa-kits |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into
aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | Artemin supports the survival of sensory and sympathetic peripheral neurons in
culture and also supports the survival of dopaminergic neurons of the ventral
mid-brain. Strong attractant of gut hematopoietic cells thus promoting the
formation Peyer's patch-like structures, a major component of the
gut-associated lymphoid tissue. Ligand for the GFR-alpha-3-RET receptor complex
but can also activate the GFR-alpha-1-RET receptor complex (UniProt) |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
Buy now
€323 / 100µg
€1292 / 500µg
€2098 / 1000µg
A
B
Figure
1.
Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with
GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin.
Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin
antibody clone 2G7. Goat anti-mouse Dylight 488 conjugated antibody (dilution
1:1000) was used as secondary antibody. For nuclear staining DAPI was used.
ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x
objective). Composite picture was generated using pseudocolors green for Artemin
and blue for nuclei. A. Artemin
bound to the GFRa3-Ret51 receptor complex; B.
Negative control - GFRa3-Ret51 receptor expressing U2OS cells
Figure 2. Immunohistochemistry testing of anti-ARTN monoclonal antibody 2G7. Analysis was performed using FFPE human cerebral cortex tissue sections from Alzheimer's disease patients. Tissue sections were boiled with sodium citrate buffer (pH 6) for antigen retrieval. Incubation with primary antibody at 5 µg/ml was performed overnight at 4°C. DAB was used for visualization. Sections were counterstained with toluidine blue.