Mouse monoclonal antibody to human Artemin
324-100
Datasheet as| Name | Mouse
monoclonal antibody to human Artemin |
| Immunogen | Human Artemin |
| Immunogen Description | Recombinant human Artemin protein produced using
CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
Immunogen is purified from cell culture supernatant |
| Uniprot ID | Q5T4W7 |
| Alternative Names | Enovin, Neublastin |
| Clonality | Mouse monoclonal |
| Clone | 2D12 |
| Class | IgG1 |
| Reactivity | Human Artemin |
| Application | ELISA, WB, IF |
| Protocol | ELISA 0.2 to 2 µg/ml; WB 0.5 to 2 µg/ml (detects only dimeric protein in non-reducing conditions); IF 0.3 to 20 µg/ml. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Monoclonal and polyclonal antibodies and ELISA kit to human Artemin. For more information visit www.icosagen.com/antibodies or http://icosagen.com/products/elisa-kits |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into
aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | Artemin supports the survival of sensory and sympathetic peripheral neurons in
culture and also supports the survival of dopaminergic neurons of the ventral
mid-brain. Strong attractant of gut hematopoietic cells thus promoting the
formation Peyer's patch-like structures, a major component of the
gut-associated lymphoid tissue. Ligand for the GFR-alpha-3-RET receptor complex
but can also activate the GFR-alpha-1-RET receptor complex (UniProt) |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
Figure
1.
Western Blot testing of purified
recombinant Artemin by anti-Artemin antibody 2D12. Goat anti-mouse IgG HRP
conjugate was used as secondary antibody. 15 ml
of CHOEBNALT85 Artemin expressing
supernatant was loaded per line. Line 1. Reduced conditions; Line 2.
Non-reduced conditions; Line 3. Protein marker (8003, Naxo)
A
B
Figure
2.
Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin. Human U2OS cells were transfected with
GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin.
Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin
antibody clone 2D12. Goat anti-mouse Dylight 488 conjugated antibody (dilution
1:1000) was used as secondary antibody. For nuclear staining DAPI was used.
ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x
objective). Composite picture was generated using pseudocolors green for Artemin
and blue for nuclei. A. Artemin
bound to the GFRa3-Ret51 receptor complex; B.
Negative control - GFRa3-Ret51 receptor expressing U2OS cells