Antibodies

Mouse monoclonal antibody to human Artemin

323-100

Datasheet as
Name Mouse monoclonal antibody to human Artemin
Immunogen Human Artemin
Immunogen Description Recombinant human Artemin protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Uniprot ID Q5T4W7
Alternative Names Enovin, Neublastin
Clonality Mouse monoclonal
Clone 2A5
Class IgG1
Reactivity Human Artemin
Application ELISA, WB, IF
Protocol

ELISA 0.2 to 1 µg/ml; WB 0.5 to 2 µg/ml (detects only dimeric protein in non-reducing conditions); IF 0.3 to 20 µg/ml. 

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Monoclonal and polyclonal antibodies and ELISA kit to human Artemin.  For more information visit www.icosagen.com/antibodies or  http://icosagen.com/products/elisa-kits 
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background Artemin supports the survival of sensory and sympathetic peripheral neurons in culture and also supports the survival of dopaminergic neurons of the ventral mid-brain. Strong attractant of gut hematopoietic cells thus promoting the formation Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Ligand for the GFR-alpha-3-RET receptor complex but can also activate the GFR-alpha-1-RET receptor complex (UniProt)
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg
Figure 1. Western Blot testing of purified recombinant Artemin by anti-Artemin antibody 2A5. For analysis HRP-conjugated 2A5 antibody was used. 200 ng of purified recombinant Artemin expressed by CHOEBNALT85 cell line was loaded per lane. Line 1. Reduced conditions; Line 2. Non-reduced conditions; Line 3. Protein marker (8003, Naxo)
A

B

Figure 2. Immunofluorescence analysis of GFRA3-Ret51 receptor complex bound Artemin.  Human U2OS cells were transfected with GFRa3-Ret51 expression vector followed by treatment with 100 nM Artemin. Artemin bound to the GFRa3-Ret51 receptor complex was detected by anti-Artemin antibody clone 2A5. Goat anti-mouse Dylight 488 conjugated antibody (dilution 1:1000) was used as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for Artemin and blue for nuclei. A. Artemin bound to the GFRa3-Ret51 receptor complex; B. Negative control - GFRa3-Ret51 receptor expressing U2OS cells