Antibodies

Monoclonal antibody to SARS-CoV-2 Spike RBD

R1-157-100

Datasheet as
Name Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12
Immunogen SARS-CoV-2 virus
Immunogen Description SARS-CoV-2 virus (PBMCs isolated from patients recovered from SARS-CoV-2 infection, antibodies discovered by using HybriFree Technology)
Clonality Recombinant human monoclonal
Clone 27B12
Class Human IgG1-kappa
Reactivity SARS-CoV-2 Spike RBD
Dissociation constant (KD) < 1.0 x 10-12 M (measured against SARS-CoV-2 Trimeric Spike protein)
2.02 x 10-8 M (measured against SARS-CoV-2 Spike S1 protein)
Application ELISA
Protocol Indirect ELISA 0.02 – 0.04 ng/ml
Purification Protein A affinity chromatography following desalting
Buffer PBS pH 7.4
Unit Size 100 µg
Shipping Shipped on dry ice
Storage Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.
Limitations This product is for research use only.
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€300 / 100µg
€500 / 500µg
€745 / 1000µg

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Figure 1. Coomassie-stained SDS-PAGE analysis of monoclonal antibody to SARS-CoV-2 Spike
RBD, clone 27B12. 4-12% gradient gel is used for analysis. Lane 1. Monoclonal antibody to SARS-CoV-2 Spike RBD, clone 27B12 (-DTT). Lane 2. Size Marker. Lane 3. Monoclonal antibody to SARS-CoV-2 Spike RBD,
clone 27B12 (+DTT). Lane 4. Size Marker