Antibodies

Chicken Anti-SARS-CoV-2 NP polyclonal antibody

A2-350-100

Datasheet as
Name Chicken Anti-SARS-CoV-2 NP polyclonal antibody
Immunogen SARS-CoV-2 Nucleocapsid protein
Immunogen Description Recombinant SARS-CoV-2 Nucleocapsid protein produced by CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line
Uniprot ID YP_009724397.2
Clonality Chicken polyclonal
Class IgY
Reactivity SARS-CoV-2 Nucleocapsid protein
Application ELISA, WB
Protocol

ELISA: 0,02-0,1  µg\ml

WB: 10 µg/ml
Purification PEG sedimention following affinity purification
Buffer Concentrated ammonium sulphate in PBS pH 7.4
Unit Size 100  µg
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use.
Limitations This product is for research use only.
Figure 1. Western Blot analysis of  Anti-SARS-CoV-2 NP polyclonal antibody. Lane 1. 20 ng of N-protein was loaded. Lane 2. 50 ng of N-protein was loaded. Lane 3. 100 ng of N-protein was loaded. Lane 4. Protein size marker. 1:200 primary antibody dilution was used for protein detection. HRP-conjugated rabbit anti-chicken IgY antibody (1:5000) was used (ab6753).