Antibodies

Chicken polyclonal antibody to human GFRa-4

308-100

Datasheet as
Name Chickenpolyclonal antibody to human GFRa-4
Immunogen human GFRa-4
Immunogen Description Recombinant His-tagged human GFRa-4 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-4, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Uniprot ID Q9GZZ7
Alternative Names GDNF receptor alpha-4, Persephin receptor
Clonality Chicken polyclonal
Class IgY
Reactivity Human GFRa-4, doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-3
Application ELISA, WB, IF
Protocol

WB 0.1 to 0.2 µg/ml; ELISA: 3 to 6.7 ng/ml; IF 0.3 to 1 µg/ml

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification GFRa-4 affinity purified
Buffer PBS pH 7.4
Unit Size 100 µg
Related Products Mono- and polyclonal antibodies to human neural growth factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Background GFRα-4 belongs to the GDNF receptor family. It is a glycosyl-phosphatidylinositol (GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor
Limitations This product is for research use only
Buy now
€299 / 100µg

Figure 1. IF detection of human GFRα-4 expressed in U2OS cells. GFRα-4 was visualized using Chicken polyclonal antibody to human GFRα-4 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-4 specific signal and blue for nuclei

Figure 2. Western-Blot detection of human GFRα-4, using Chicken polyclonal antibody to human GFRα-4. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. hGFRα-4. Primary antibody dilution 1:5000 was used. Line 2. Prestained protein ladder (Naxo 8003). Rabbit anti-chicken antibody, HRP conjugate (903-100, Icosagen) was used as secondary antibody