Antibodies

Rabbit anti-chicken polyclonal antibody HRP conjugate

903-100

Datasheet as
Name Rabbit anti-chicken polyclonal antibody HRP conjugate
Immunogen chicken IgY
Immunogen Description Purified IgY fraction
Clonality Rabbit polyclonal, HRP conjugate
Class IgG
Reactivity Reacts with chicken IgY antibodies
Application ELISA, WB
Protocol

ELISA: 0.03 to 0.05 µg/ml, WB: 0.1 to 0.2 µg/ml

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification IgG fraction purified by IgY affinity chromatography
Buffer PBS pH 7.4, with 50% glycerol
Unit Size 100 µg
Related Products Rabbit-anti chicken polyclonal antibodies, different conjugates. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in frozen format
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Limitations This product is for research use only
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€299 / 100µg























Figure 1. Western blot analysis for testing of Rabbit anti-chicken polyclonal antibody HRP conjugate as secondary antibody. Chicken anti-MANF polyclonal antibody (Icosagen) was used as primary antibody.

For secondary antibody testing purified human MANF protein was used. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified MANF protein. 100 ng of protein is loaded per lane