Mouse monoclonal antibody to human Neurturin
322-100
Datasheet as| Name | Mouse
monoclonal antibody to human Neurturin |
| Immunogen | human Neurturin |
| Immunogen Description | Recombinant human NRTN protein produced using
CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Protein
was purified from the cell culture supernatant |
| Uniprot ID | Q99748 |
| Alternative Names | NRTN, NTN |
| Clonality | Mouse Monoclonal |
| Clone | 1B11 |
| Class | IgG1 |
| Reactivity | Human NRTN |
| Application | ELISA, WB, IF |
| Protocol |
WB 0.2 to 1 µg/ml; ELISA: 0.05 to 0.1 µg/ml; IF 5 to 10 µg/ml Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Mono- and polyclonal antibodies to human growth
factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at – 20 or -70 °C upon receipt. Divide antibody
into aliquots prior usage. Avoid multiple freeze-thaw cycles as product
degradation may result |
| Background | Neurturin is a member of the GDNF family of ligands.
GDNF family proteins are distant members of the TGF-beta superfamily |
| References | - |
| Limitations | This
product is for research use only |
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€299 / 100µg
Figure 1. Western-Blot detection of human NRTN expressed in CHO cells. Line 1. hNRTN-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 µl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. AP-conjugated Goat-anti mouse, was used as secondary antibody. Antibody recognizes protein dimeric, and monomeric variants