Rabbit polyclonal antibody to human Neurturin
307-100
Datasheet as| Name | Rabbit
polyclonal antibody to human Neurturin |
| Immunogen | human Neurturin |
| Immunogen Description | Recombinant human NRTN protein produced using
CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Protein
was purified from the cell culture supernatant |
| Uniprot ID | Q99748 |
| Alternative Names | NRTN, NTN |
| Clonality | Rabbit polyclonal |
| Clone | - |
| Class | IgG |
| Reactivity | Human NRTN |
| Application | ELISA, WB, IF |
| Protocol |
WB 0.2 to 1 µg/ml; ELISA: 0.01 to 0.02 µg/ml; IF 0.5 to 2 µg/ml Antibody doesn’t cross-react to other GDNF-family proteins Polyclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | Immunogen affinity purification |
| Buffer | Concentrated ammonium sulphate in PBS pH 7.4 |
| Unit Size | 100 µg |
| Related Products | Mono- and polyclonal antibodies to human growth
factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at +4°C upon receipt. As product is (NH4)2SO4
precipitate, mix well by pipetting or vortexing prior use |
| Background | Neurturin (NTN) is a member of the GDNF family of
ligands. GDNF family proteins are distant members of the TGF-beta superfamily |
| References | - |
| Limitations | This
product is for research use only |
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€323 / 100µg
€1292 / 500µg
€2098 / 1000µg
Figure 1. Western-Blot detection of human NRTN expressed in CHO cells. Line 1. hNRTN-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 µl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rb pAb to Chk IgY ab6753, was used as secondary antibody. Antibody recognizes protein dimeric, and monomeric variants
