Antibodies

Rabbit polyclonal antibody to human Neurturin

307-100

Datasheet as
Name Rabbit polyclonal antibody to human Neurturin
Immunogen human Neurturin
Immunogen Description Recombinant human NRTN protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Protein was purified from the cell culture supernatant
Uniprot ID Q99748
Alternative Names NRTN, NTN
Clonality Rabbit polyclonal
Clone -
Class IgG
Reactivity Human NRTN
Application ELISA, WB, IF
Protocol

WB 0.2 to 1 µg/ml; ELISA: 0.01 to 0.02 µg/ml; IF 0.5 to 2 µg/ml

Antibody doesn’t cross-react to other GDNF-family proteins

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Immunogen affinity purification
Buffer Concentrated ammonium sulphate in PBS pH 7.4
Unit Size 100 µg
Related Products Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background Neurturin (NTN) is a member of the GDNF family of ligands. GDNF family proteins are distant members of the TGF-beta superfamily
References -
Limitations This product is for research use only
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€299 / 100µg

Figure 1. Western-Blot detection of human NRTN expressed in CHO cells. Line 1. hNRTN-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 µl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Rb pAb to Chk IgY ab6753, was used as secondary antibody. Antibody recognizes protein dimeric, and monomeric variants