Antibodies

Chicken polyclonal antibody to human RET

306-100

Datasheet as
Name Chicken polyclonal antibody to human RET
Immunogen human RET
Immunogen Description Recombinant His-tagged extracellular fragment of human RET protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. The extracellular fragment of hRET was expressed and secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Uniprot ID P07949
Alternative Names Proto-oncogene tyrosine-protein kinase receptor Ret, Cadherin family member 12,
Proto-oncogene c-Ret
Clonality Chicken polyclonal
Clone -
Class IgY
Reactivity Human RET
Application ELISA, WB, IF
Protocol

WB 0.1 to 0.2 µg/ml; ELISA: 8 to 12,5 ng/ml, IF 2 to 4 µg/ml

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification RET affinity purified
Buffer Concentrated ammonium sulphate in PBS pH 7.4
Unit Size 100 µg
Related Products Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background The RET proto-oncogene is a receptor tyrosine kinase for members of the glial cell line-derived neurotrophic factor family of extracellular signalling molecules
References -
Limitations This product is for research use only
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€299 / 100µg

Figure 1. Immunofluorescence detection of human RET expressed in U2OS cells. RET was visualized using Chicken polyclonal antibody to human RET (dilution 1:500) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for RET specific signal and blue for nuclei.


Figure 2.
Western-Blot detection of human RET expressed in CHO cells. Line 1. 100 ng human recombinant RET-His protein was loaded per line. Primary antibody dilution 0.2 µg/ml was used. Rb pAb to Chk IgY ab6753, was used as secondary antibody