Antibodies

Chicken polyclonal antibody to human GFRa-3

305-100

Datasheet as
Name Chicken polyclonal antibody to human GFRa-3
Immunogen human GFRa-3
Immunogen Description Recombinant His-tagged human GFRa-3 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Uniprot ID O60609
Alternative Names GDNF receptor alpha-3
Clonality Chicken polyclonal
Clone -
Class IgY
Reactivity Human GFRa-3,
doesn’t cross-react with hGFRa-1, hGFRa-2, hGFRa-4
Application ELISA, WB, IF
Protocol WB 1 to 2 µg/ml; ELISA: 3 to 6.25 ng/ml; IF 0.3 to 0.67 µg/ml.
Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification GFRα-3 affinity purified
Buffer Concentrated ammonium sulphate in PBS pH 7.4
Unit Size 100 µg
Related Products Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin
References -
Limitations This product is for research use only
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€299 / 100µg

Figure 1. IF detection of human GFRα-3 expressed in U2OS cells. GFRα-3 was visualized using Chicken polyclonal antibody to human GFRα-3 (dilution 1:3000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-3 specific signal and blue for nuclei.


Figure 2. Western-Blot detection of human GFRα-3, using Chicken polyclonal antibody to human GFRα-3. GFRα-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-3. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody (Icosagen)  was used as secondary antibody