Chicken polyclonal antibody to human GFRa-1
303-100
Datasheet as| Name | Chicken
polyclonal antibody to human GFRa-1 |
| Immunogen | human GFRa-1 |
| Immunogen Description | Recombinant His-tagged human GFRa-1 protein produced
using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line.
For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removed
and protein was secreted to the cell culture supernatant. Protein was purified
by Ni-affinity chromatography following gel-filtration from cell culture
supernatant |
| Uniprot ID | P56159 |
| Alternative Names | RET ligand 1, TGF-beta related neurotrophic factor
receptor 1 |
| Clonality | Chicken polyclonal |
| Clone | - |
| Class | IgY |
| Reactivity | Human GFRa-1 doesn’t cross-react with hGFRa-2, hGFRa-3, hGFRa-4 |
| Application | ELISA, WB, IF |
| Protocol | ELISA:
0.02 to 0.05 µg/ml; WB 0.1 to 0.2 µg/ml; IF 0.5 to 1 µg/ml Polyclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | GFRa-1 affinity purified |
| Buffer | Concentrated ammonium sulphate in PBS pH 7.4 |
| Unit Size | 100 µg |
| Related Products | Mono- and polyclonal antibodies to human growth
factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in
ambient conditions |
| Storage | Store at +4 °C upon receipt. As product is (NH4)2SO4
precipitate, mix well by pipetting or vortexing prior use |
| Background | Glial cell line-derived neurotrophic factor (GDNF) and
neurturin (NTN) are two structurally related, potent neurotrophic factors that
play key roles in the control of neuron survival and differentiation. The GFRa-1
is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol
(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates
activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674) |
| References | - |
| Limitations | This
product is for research use only |
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€299 / 100µg
Figure 1. Immunofluorescence detection of human GFRα-1 expressed in U2OS cells. GFRα-1 was visualized using Chicken polyclonal antibody to human GFRα-1 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-1 specific signal and blue for nuclei.
Figure 2. Western-Blot detection of human GFRα-1, using Chicken polyclonal antibody to human GFRα-1. GFRα-1 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-1. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody (Icosagen) was used as secondary antibody