Antibodies

Chicken polyclonal antibody to human GFRa-1

303-100

Datasheet as
Name Chicken polyclonal antibody to human GFRa-1
Immunogen human GFRa-1
Immunogen Description
Recombinant His-tagged human GFRa-1 protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. For production of hGFRa-1, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant
Uniprot ID P56159
Alternative Names RET ligand 1, TGF-beta related neurotrophic factor receptor 1
Clonality Chicken polyclonal
Clone -
Class IgY
Reactivity Human GFRa-1
doesn’t cross-react with hGFRa-2, hGFRa-3, hGFRa-4
Application ELISA, WB, IF
Protocol
ELISA: 0.02 to 0.05 µg/ml; WB 0.1 to 0.2 µg/ml; IF 0.5 to 1 µg/ml
Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification GFRa-1 affinity purified
Buffer Concentrated ammonium sulphate in PBS pH 7.4
Unit Size 100 µg
Related Products Mono- and polyclonal antibodies to human growth factors and neural growth factor receptors. For more details visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at +4 °C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The GFRa-1 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor (www.ncbi.nlm.nih.gov/gene/2674)
References -
Limitations This product is for research use only
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€299 / 100µg

Figure 1.
Immunofluorescence  detection of human GFRα-1 expressed in U2OS cells. GFRα-1 was visualized using Chicken polyclonal antibody to human GFRα-1 (dilution 1:2000) and Goat anti-chicken IgY - H&L DyLight 550 (Abcam, ab96952) (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-1 specific signal and blue for nuclei.


Figure 2. Western-Blot detection of human GFRα-1, using Chicken polyclonal antibody to human GFRα-1. GFRα-1 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-1. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody (Icosagen) was used as secondary antibody