Mouse monoclonal antibody to bovine DNase I
404-100
Datasheet as| Name | Mouse monoclonal antibody to bovine DNase I |
| Immunogen | Bovine DNase I |
| Immunogen Description | Recombinant full length bovine DNaseI produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Antigen is purified from cell culture supernatant |
| Uniprot ID | P00639 |
| Alternative Names | DNL1 |
| Clonality | Mouse monoclonal |
| Clone | 4B10 |
| Class | IgG1 |
| Reactivity | Bovine, cross reactivity to Human |
| Application | ELISA, WB |
| Protocol | ELISA
0.01 to 0.02 µg/ml; WB 0.1 to 0.2 μg/ml (bovine DNase I) Antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4 |
| Unit Size | 100 µg |
| Related Products | Bovine DNase I polyclonal antibody, cat no 400-100 |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20… -70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles as product degradation may result |
| Background | Bovine deoxyribonuclease I (DNase I) degrades single- and double-stranded DNA. The enzyme works by cleaving DNA into 5' phosphodinucleotide and small oligonucleotide fragments |
| Limitations | This product is for research use only |
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€299 / 100µg
Figure 1. Western Blot testing of anti-bovine DNaseI monoclonal antibody (4B10).
Lane 1. Recombinant bovine DNaseI expressed into the supernatant of CHO cell culture medium. 10 µl of cell culture supernatant was loaded into the gel. Lane 2. PageRuler Prestained Protein Ladder (#SM0671 Fermentas);