Antibodies

Mouse monoclonal antibody to HIV-1 p24

A2-851-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 p24
Immunogen HIV-1 p24
Immunogen Description Recombinant p24 protein of HIV-1 HAN consensus (purified from E. coli)
Clonality Mouse monoclonal
Clone 5
Class IgG2a
Reactivity Reacts with HIV-1 p24 subtype B, C and A. Mapped to amino acids 48-62 (TPQDLNTMLNTVGGH)
Application ELISA, WB, IF
Protocol

ELISA 0.05 to 0.1 µg/ml; WB 1 µg/ml; IF 1 µg/ml.

Antibody can be used efficiently for IF application in U2OS, CHO and 293-based cell lines.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Partially humanized, chimeric antibody to HIV-1 p25 C-151-100, clone 5. HIV-1 p24 monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1: Western-Blot analysis of HIV-1 HAN subtype gag protein using HIV-1 p24 (05-001) antibody. Line 1. Recombinant HIV-1 HAN-2 subtype gag expressed in composition of virus like particles (VLPs) using 293-based cell line. Line 2. Protein size marker



Figure 2: IF analysis using HIV-1 p24 antibody (05-001) in detection of 05-001 epitope tagged ETAR (Endothelin A receptor) in HEK293-based cell line