Mouse monoclonal antibody to HIV-1 p24
A2-851-100
Datasheet as| Name | Mouse monoclonal antibody to HIV-1 p24 |
| Immunogen | HIV-1 p24 |
| Immunogen Description | Recombinant p24 protein of HIV-1 HAN consensus (purified from E. coli) |
| Clonality | Mouse monoclonal |
| Clone | 5 |
| Class | IgG2a |
| Reactivity | Reacts with HIV-1 p24 subtype B, C and A. Mapped to amino acids 48-62 (TPQDLNTMLNTVGGH) |
| Application | ELISA, WB, IF |
| Protocol |
ELISA 0.05 to 0.1 µg/ml; WB 1 µg/ml; IF 1 µg/ml. Antibody can be used efficiently for IF application in U2OS, CHO and 293-based cell lines. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Partially humanized, chimeric antibody to HIV-1 p25 C-151-100, clone 5. HIV-1 p24 monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles. |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
Figure 1: Western-Blot analysis of HIV-1 HAN subtype gag protein using HIV-1 p24 (05-001) antibody. Line 1. Recombinant HIV-1 HAN-2 subtype gag expressed in composition of virus like particles (VLPs) using 293-based cell line. Line 2. Protein size marker
