Antibodies

Partially humanized, chimeric recombinant monoclonal antibody to HIV-1 p24

C-851-100

Datasheet as
Name Partially humanized, chimeric recombinant monoclonal antibody to HIV-1 p24
Immunogen HIV-1 p24
Immunogen Description Recombinant p24 protein of HIV-1 HAN consensus (purified from E. coli)
Clonality Partially humanized monoclonal
Clone 5
Class IgG1
Reactivity Reacts with HIV-1 p24 subtype B, C and A. Mapped to 48-62(TPQDLNTMLNTVGGH)
Application ELISA, WB
Protocol

WB 1 µg/ml; IF 1 to 10 µg/ml

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

For detection please use anti-human IgG antibody
Purification Protein G purification
Buffer PBS pH 7.4, 50% glycerole
Unit Size 100 µg
Related Products Mouse anti-HIV 1 p24 monoclonal antibody clone 5 A2-851-100. HIV-1 p24 monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background Antibody is derived from mouse anti-HIV-1 (HAN subtype) p17/24 monoclonal antoibody clone 5 expressing mouse hybridoma culture. cDNA of antibody variable regions were isolated and cloned into the human IgG1 constant region-containing antibody expression vector. Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cell line. Antibody is used for high-specific efficient detection of soluble or membrane-bound proteins fused with 05-001 epitope-tag sequence. For more information please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1: Western-Blot analysis of HIV-1 HAN subtype p17/24 protein using chimeric HIV-1 p24 antibody, clone 5. Line 1. Protein size marker Line 2. HIV-1 p17/24 20 ng per lane

Figure 2: IF analysis using chimeric HIV-1 p24 antibody, clone 5. ETAR (Endothelin A receptor) is fused with 05-001 epitope tag, expressed in  CHOEBNALT85 cell line and detected in IF assay