Antibodies

Rabbit polyclonal antibody to MLV p30

A1-910-100

Datasheet as
Name Rabbit polyclonal antibody to MLV p30
Immunogen MLV p30
Immunogen Description Full length recombinant MLV-Gag p30 capside protein, purified from E. coli
Clonality Rabbit polyclonal
Class IgG
Reactivity MLV Gag, p30
Application ELISA, WB
Protocol

ELISA 0.01 ug/ml; WB 0.5 - 1 µg/ml

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification MLV p30 affinity purified
Buffer Concentrated ammonium sulphate in PBS pH 7.4.
Unit Size 100 µg
Related Products Monoclonal and polyclonal antibodies to MLV p30.  For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background Murine leukemia viruses belong to family of Retroviruses group and are named for their ability to cause cancer in mice. Some form of MLV’s are used in cancer research. The retroviral Gag protein plays important roles at several stages in the viral life cycle. Late in infection, the Gag precursor protein acts to mediate the assembly and release of virion particles. After budding, the core matures by cleavage of Gag precursor protein into the mature core proteins, the matrix (MA), capsid (CA) and nucleocapsid proteins. These separated Gag proteins then play roles in the early stages of infection
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

Buy now
€299 / 100µg

Figure 1. Western Blot testing of MLV-gag antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas); Line 2. HEK293 cells; Line 3. MLV-gag protein expressed in HEK293 cells; Line 4. PageRuler Prestained Protein Ladder (#SM0671, lot: 00052778, Fermentas); Line 5. CHO cells; Line 6. MLV-gag protein expressed in CHO cells. 10 000 cells were lysed and loaded into each lane.