Antibodies

Rabbit polyclonal antibody to human MANF

310-100

Datasheet as
Name Rabbit polyclonal antibody to human MANF
(Mesencephalic astrocyte-derived neurotrophic factor)
Immunogen Human MANF
Immunogen Description Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Uniprot ID P55145
Alternative Names ARMET, ARP
Clonality Rabbit polyclonal
Clone -
Class IgG
Reactivity Human MANF
Application ELISA, WB, IF, IHC
Protocol

ELISA 0.1 to 0.2 µg/ml; WB 0.15 to 0.5 µg/ml, IHC 10 µg/ml, IF 1 to 20 µg/ml

No cross-reactivity in MANF-knockout mouse. No cross-reactivity with CDNF in IHC, slightly cross-reacts with purified CDNF in WB.  

Polyclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification MANF-affinity purified
Buffer Concentrated ammonium sulphate in PBS pH 7.4.
Unit Size 100 µg
Related Products Monoclonal and polyclonal antibodies to human MANF.  For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Storage: Store at +4°C upon receipt. As product is (NH4)2SO4 precipitate, mix well by pipetting or vortexing prior use
Background MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
References -
Limitations This product is for research use only
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€299 / 100µg

Figure 1. Western Blot testing of anti-MANF polyclonal antibody. Line 1. PageRuler Prestained Protein Ladder (#SM0671 Fermentas); Line 2. Purified recombinant MANF (1 µg per line) expressed using CHOEBNALT85  cell line

A.

B.

Figure 2: Immunohistochemical analysis of rabbit polyclonal antibody to human MANF. Analysis was performed using 4-5 µm FFPE human cerebral cortex tissue sections from Alzheimer’s disease patients. Antibody concentration of 10 µg/ml was used. A. MANF staining by polyclonal antibody. B. negative staining.

A.                         B.

Figure 3. Immunofluorescence detection of human MANF expressed in human U2OS cells by rabbit polyclonal antibody to hMANF. Anti-hMANF antibody dilution in IF experiment was 1 µg/ml. A. MANF-expressing U2OS cells. B. negative control (non-transfected cells)