Mouse monoclonal antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor)
311-100
Datasheet as| Name | Mouse monoclonal antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor) |
| Immunogen | Human MANF |
| Immunogen Description | Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant |
| Uniprot ID | P55145 |
| Alternative Names | ARMET, ARP |
| Clonality | Mouse monoclonal |
| Clone | 1G12 |
| Class | IgG1 |
| Reactivity | Human MANF |
| Application | ELISA, WB, IF |
| Protocol |
ELISA 0.1 to 0.2 µg/ml; WB 0.5 to 2 µg/ml; IF 1 to 10 µg/ml |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Monoclonal and polyclonal antibodies to human MANF. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20°C to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta |
| References | - |
| Limitations | This product is for research use only |
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€299 / 100µg
Figure 1. Western Blot testing of anti-MANF monoclonal antibody. Line 1. Page Ruler prestained protein ladder, Fermentas; Line 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85 cell line