Mouse monoclonal antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor)
311-100
Datasheet asName | Mouse monoclonal antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor) |
Immunogen | Human MANF |
Immunogen Description | Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant |
Uniprot ID | P55145 |
Alternative Names | ARMET, ARP |
Clonality | Mouse monoclonal |
Clone | 1G12 |
Class | IgG1 |
Reactivity | Human MANF |
Application | ELISA, WB, IF |
Protocol |
ELISA 0.1 to 0.2 µg/ml; WB 0.5 to 2 µg/ml; IF 1 to 10 µg/ml |
Purification | Protein G purification |
Buffer | PBS pH 7.4, with 0.1% sodium azide |
Unit Size | 100 µg |
Related Products | Monoclonal and polyclonal antibodies to human MANF. For more information visit www.icosagen.com/antibodies |
Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
Storage | Store at -20°C to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
Background | MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta |
References | - |
Limitations | This product is for research use only |
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Price per quantity:
€308 / 100µg
€1231 / 500µg
€1999 / 1000µg
Figure 1. Western Blot testing of anti-MANF monoclonal antibody. Line 1. Page Ruler prestained protein ladder, Fermentas; Line 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85 cell line