Antibodies

Mouse monoclonal antibody to human MANF (Mesencephalic astrocyte-derived neurotrophic factor)

311-100

Datasheet as
Name Mouse monoclonal antibody to human MANF
(Mesencephalic astrocyte-derived neurotrophic factor)
Immunogen Human MANF
Immunogen Description Recombinant human MANF protein produced using CHO-based Icosagen Cell factory Ltd. proprietary suspension cell line. Immunogen is purified from cell culture supernatant
Uniprot ID P55145
Alternative Names ARMET, ARP
Clonality Mouse monoclonal
Clone 1G12
Class IgG1
Reactivity Human MANF
Application ELISA, WB, IF
Protocol

ELISA 0.1 to 0.2 µg/ml; WB 0.5 to 2 µg/ml; IF 1 to 10 µg/ml
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Monoclonal and polyclonal antibodies to human MANF.  For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20°C  to -70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta
References -
Limitations This product is for research use only
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€299 / 100µg

Figure 1. Western Blot testing of anti-MANF monoclonal antibody. Line 1. Page Ruler prestained protein ladder, Fermentas; Line 2. Purified recombinant MANF (500 ng per line) expressed using CHOEBNALT85  cell line