Antibodies

Mouse monoclonal antibody to human laminin a4

A2-604-100

Datasheet as
Name Mouse monoclonal antibody to human laminin a4
Immunogen Human laminin a4
Immunogen Description Immunoaffinity-purified Laminin 411 (laminin-8 (α4β1γ1) from platelets
Uniprot ID Q16363
Alternative Names -
Clonality Mouse monoclonal
Clone 6C3
Class IgG1
Reactivity Human laminin α4
Application ELISA, WB, IP
Protocol

ELISA titer 0.25 µg/ml; WB in reduced conditions; 0.5 to 1 µg/ml; IP 0.5 mg/ml

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products Monoclonal antibodies to human laminin.  For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination,laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their α chain, are expressed in a cell- and tissue-specific manner, and are differentially recognized by integrins
References

Wondimu Z, Geberhiwot T, Ingerpuu S, Juronen E, Xie X, Lindbom L, Doi M, Kortesmaa J, Thyboll J, Tryggvason K, Fadeel B, Patarroyo M. (2004) An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. Blood, 104(6):1859-66.

Kawataki T, Yamane T, Naganuma H, Rousselle P, Andurén I, Tryggvason K, Patarroyo M. (2007)

Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. Exp Cell Res
Limitations This product is for research use only
Buy now
€299 / 100µg

Figure 1. Reactivity of laminin α4 chain specific monoclonal antibody 6C3 on human platelet lysate by Western blotting (reducing, R and nonreducing, NR conditions).