Mouse monoclonal antibody to human laminin a4
A2-604-100
Datasheet as| Name | Mouse monoclonal antibody to human laminin a4 |
| Immunogen | Human laminin a4 |
| Immunogen Description | Immunoaffinity-purified Laminin 411 (laminin-8 (α4β1γ1) from platelets |
| Uniprot ID | Q16363 |
| Alternative Names | - |
| Clonality | Mouse monoclonal |
| Clone | 6C3 |
| Class | IgG1 |
| Reactivity | Human laminin α4 |
| Application | ELISA, WB, IP |
| Protocol |
ELISA titer 0.25 µg/ml; WB in reduced conditions; 0.5 to 1 µg/ml; IP 0.5 mg/ml Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | Monoclonal antibodies to human laminin. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| Background | Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination,laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their α chain, are expressed in a cell- and tissue-specific manner, and are differentially recognized by integrins |
| References |
Wondimu Z, Geberhiwot T, Ingerpuu S, Juronen E, Xie X, Lindbom L, Doi M, Kortesmaa J, Thyboll J, Tryggvason K, Fadeel B, Patarroyo M. (2004) An endothelial laminin isoform, laminin-8 (α4β1γ1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis. Blood, 104(6):1859-66. Kawataki T, Yamane T, Naganuma H, Rousselle P, Andurén I, Tryggvason K, Patarroyo M. (2007) Laminin isoforms and their integrin receptors in glioma cell migration and invasiveness: Evidence for a role of alpha5-laminin(s) and alpha3beta1 integrin. Exp Cell Res |
| Limitations | This product is for research use only |
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€299 / 100µg
Figure 1. Reactivity
of laminin α4 chain specific monoclonal antibody 6C3 on human platelet lysate
by Western blotting (reducing, R and nonreducing, NR conditions).