Antibodies

Mouse monoclonal antibody to HPV-18 E7

A2-406-100

Datasheet as
Name Mouse monoclonal antibody to HPV-18 E7
Immunogen HPV-18 E7
Immunogen Description Recombinant full-length HPV-18 E7-protein purified from E. coli
Clonality Mouse monoclonal
Clone 8E2
Class IgG1
Reactivity Reacts with HPV-18 E7-protein.  Mapped to amino acids 36-70
Application ELISA, WB, IF, IP
Protocol

ELISA 0.05 – 0.1 µg/ml ; WB 1 µg/ml;  IF 0.33 – 10 µg/ml;  IP 2 µg/ml.

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HPV-11 and HPV-18 E7 monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
Background Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins have cellular transforming and trans-activating activities
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. Western-Blot analysis of HPV-18 E7 protein. WB was carried out with 8E2 Mab using the lysates of U20S, Hela and SiHa cells. Line 1. U20S with plasmid expressing E7 protein, Line 2. plain U2OS cells. Line 3. Lysate of Hela cells. Hela cells are containing HPV-18 genome sequences expressing HPV E6 and E7 proteins. Line 4. Lysate of SiHa cells. SiHa cells are containing HPV-16 genome sequence, Line 5. Protein size marker


Figure 2. IF analysis of HPV-18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus-localized HPV-18 E7 detected by Mab 8E2. IF analysis of E7 protein show similar result also in HeLa cells


Figure 3.  IP analysis of HPV-18 E7 protein.  Lane 1: detection of E7 protein, tagged by BPV epitope tag 5E11 by Mab 8E2 in lysate of U2OS cells. Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of Mab. Lane 3: precipitation without mab 8E2. Lanes 4 and 5: IP exp. with non-transfected U2OS and HeLa cells, respectively. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells. Lane 6: marker