Antibodies

Mouse monoclonal antibody to HPV-11 E2

A2-403-100

Datasheet as
Name Mouse monoclonal antibody to HPV-11 E2
Immunogen HPV-11 E2
Immunogen Description Recombinant full-length HPV-11 E2-protein purified from E. coli
Clonality Mouse monoclonal
Clone 10E12
Class IgG1
Reactivity Reacts with HPV-11 native E2-protein.  Mapped to amino acids 202-284
Application ELISA, WB, IF
Protocol ELISA 0.05 to 0.1 µg/ml; WB 1 µg/ml
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
This antibody does not work in IP
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HPV-11 and HPV-18 E2 monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg

Figure 1. Western-Blot analysis of HPV-11 E2 protein. WB was carried out with 10E12 Mab using the lysates of U20S and Hela cells. Lines 1 and 4 are according cells transfected with plasmid expressing HPV-11  E2 protein, lines 2 and 3 Non-transfected   U2OS and Hela cells. Line 5.  Protein size marker

Figure 2. IF analysis of HPV-11 E2 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-11 E2 protein. All the nuclei were stained by DAPI.  Green color represents nucleus-localized HPV-11 E2 detected by Mab 10E12. IF analysis of E2 protein show similar result also in HeLa cells