Mouse monoclonal antibody to SIV-1 Nef
A2-817-100
Datasheet as| Name | Mouse monoclonal antibody to SIV-1 Nef |
| Immunogen | SIV-1 Nef |
| Immunogen Description | Recombinant SIV-1 Mac251 Nef protein of (purified from E. coli) |
| Clonality | Mouse monoclonal |
| Clone | 17 |
| Class | IgG1 |
| Reactivity | Reacts in ELISA, WB, RIP, IHC with Nef of SIVmac251
strain. (Piguet 1998, Khan 1998,
Guillemin 2000). Reacts in IF with Nef of SIVmac239 strain and SIVmac1A11
strain. (Khan 1998, Chan 2005). Mapped to amino acids 71-80 (-PAEEREKLAY-, SIV-1) |
| Application | ELISA, WB, IHC, RIP |
| Protocol | ELISA titer: 0.05 to 0.1 µg/ml, WB: 1 µg/ml. Antibody working titer has to be established practically for each particular antigen and assay
format. |
| Purification | Protein G purification |
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| References |
Khan IH, Sawai ET, Antonio E, Weber CJ, Mandell CP, Montbriand P, Luciw PA. (1998) Role of the SH3-Ligand Domain of Simian Immunodeficiency Virus Nef in Interaction with Nef-Associated Kinase and Simian AIDS in Rhesus Macaques. J. Virol. 72: 5820-30 Piguet V, Chen Y-L, Mangasarian A, Foti M, Carpentier J-L, Trono D. (1998) Mechanism of Nef-induced CD4 endocytosis: Nef connects CD4 with the µ chain of adaptor complexes. EMBO, 17 (9): 2472-81. Mandell, CP, Reyes RA, Cho K, Sawai ET, Fang AL, Schmidt KA, Luciw PA. (1999) SIV/HIV Nef recombinant virus (SHIVnef) produces simian AIDS in rhesus macaques. Virology 265(2):235-51. Sawai ET, Hamza MS, Ye M, Shaw KE, Luciw PA. (2000) Pathogenic Conversion of Live Attenuated Simian Immunodeficiency Virus Vaccines Is Associated with Expression of Truncated Nef. Journal of Virology, 74(4): 2038-45. Guillemin G, Croitoru J, Le-Grand R, Franck-Duchenne M, Dormont D, Boussin F D. (2000) Simian immunodeficiency virus mac251 infection of astrocytes. J of Neurovirology 6(3): 173-86. Chan KS, Verardi PH, Legrand FA, Yilma TD.(2005) Nef from pathogenic simian immunodeficiency virus is a negative factor for vaccinia virus. PNAS 102(24): 8734-9. |
| Limitations | This product is for research use only |
