Antibodies

Mouse monoclonal antibody to SIV-1 Nef

A2-817-100

Datasheet as
Name Mouse monoclonal antibody to SIV-1 Nef
Immunogen SIV-1 Nef
Immunogen Description Recombinant SIV-1 Mac251 Nef protein of (purified from E. coli)
Clonality Mouse monoclonal
Clone 17
Class IgG1
Reactivity Reacts in ELISA, WB, RIP, IHC with Nef of SIVmac251 strain. (Piguet 1998, Khan 1998, Guillemin 2000). Reacts in IF with Nef of SIVmac239 strain and SIVmac1A11 strain. (Khan 1998, Chan 2005).
Mapped to amino acids 71-80 (-PAEEREKLAY-, SIV-1)
Application ELISA, WB, IHC, RIP
Protocol ELISA titer: 0.05 to 0.1 µg/ml, WB: 1 µg/ml. 
Antibody working titer has to be established practically for each particular antigen and assay format.
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References

Khan IH, Sawai ET, Antonio E, Weber CJ, Mandell CP, Montbriand P, Luciw PA. (1998) Role of the SH3-Ligand Domain of Simian Immunodeficiency Virus Nef in Interaction with Nef-Associated Kinase and Simian AIDS in Rhesus Macaques. J. Virol. 72: 5820-30  

Piguet V, Chen Y-L, Mangasarian A, Foti M, Carpentier J-L, Trono D. (1998) Mechanism of Nef-induced CD4 endocytosis: Nef connects CD4 with the µ chain of adaptor complexes. EMBO, 17 (9): 2472-81.

Mandell, CP, Reyes RA, Cho K, Sawai ET, Fang AL, Schmidt KA, Luciw PA. (1999) SIV/HIV Nef recombinant virus (SHIVnef) produces simian AIDS in rhesus macaques. Virology 265(2):235-51.

Sawai ET, Hamza MS, Ye M, Shaw KE, Luciw PA. (2000) Pathogenic Conversion of Live Attenuated Simian Immunodeficiency Virus Vaccines Is Associated with Expression of Truncated Nef. Journal of Virology, 74(4): 2038-45.

Guillemin G, Croitoru J, Le-Grand R, Franck-Duchenne M, Dormont D, Boussin F D. (2000) Simian immunodeficiency virus mac251 infection of astrocytes. J of Neurovirology 6(3): 173-86.

Chan KS, Verardi PH, Legrand FA, Yilma TD.(2005) Nef from pathogenic simian immunodeficiency virus is a negative factor for vaccinia virus. PNAS 102(24): 8734-9.
Limitations This product is for research use only
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€299 / 100µg
Figure 1. Western Blot testing of SIV-1 Nef antibody (clone 17). Different amounts (100ng, 10ng and 1ng) of SIVmac239-nef-his protein was loaded into the gel. Lanes 1-3: with DTT; Lane 4: Protein size marker; Lanes 5-7: without DTT