Antibodies

Mouse monoclonal antibody to HIV-1 Tat

A2-827-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Tat
Immunogen HIV-1 Tat
Immunogen Description Recombinant Tat protein of HIV-1 HAN (purified from E. coli)
Clonality Mouse monoclonal
Clone N8
Class IgG2b
Reactivity Reacts in ELISA and WB with HIV-1 Tat of HXB2, BH10, IIIB and HAN strains. Mapped to amino acids 59-67 (PQDSQTHQV – B subtype a.a. consensus)
Application ELISA, WB, ChIP
Protocol ELISA 0.05 to 0.1 µg/ml; WB 2 µg/ml. 
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70 °C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg













Figure 1. Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone N8). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size marker


Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone N8). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA