Antibodies

Mouse monoclonal antibody to HIV-1 Tat

A2-825-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Tat
Immunogen HIV-1 Tat
Immunogen Description Recombinant Tat protein of HIV-1 HXB2  (purified from E. coli)
Clonality Mouse monoclonal
Clone N3
Class IgM
Reactivity Reacts with HIV-1 Tat of the strains HXB2, BH10, IIIB and HAN. Mapped to amino acids 47-58 (YGKKRRQRRRA – B subtype a.a. consensus, Tat basic domain)
Application ELISA, WB, ChIP
Protocol ELISA 5 µg/ml; WB 2 µg/ml 
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification HiTrap IgM purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References Jung HJ, Park Y, Hahm KS, Lee DG.(2006) Biological activity of Tat (47-58) peptide on human pathogenic fungi. Biochem Biophys Res Commun.  345: 222-8.
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg













Figure 1:  Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone N3). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml.  Lane 2. Protein size marker


Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone N3). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA