Mouse monoclonal antibody to HIV-1 Tat
A2-825-100
Datasheet as| Name | Mouse monoclonal antibody to HIV-1 Tat |
| Immunogen | HIV-1 Tat |
| Immunogen Description | Recombinant Tat protein of HIV-1 HXB2 (purified from E. coli) |
| Clonality | Mouse monoclonal |
| Clone | N3 |
| Class | IgM |
| Reactivity | Reacts with HIV-1 Tat of the strains HXB2, BH10, IIIB and HAN. Mapped to amino acids 47-58 (YGKKRRQRRRA – B subtype a.a. consensus, Tat basic domain) |
| Application | ELISA, WB, ChIP |
| Protocol | ELISA
5 µg/ml; WB 2 µg/ml Monoclonal antibody working titer has to
be established practically for each particular antigen and assay format |
| Purification | HiTrap IgM purification
|
| Buffer | PBS pH 7.4, with 0.1% sodium azide |
| Unit Size | 100 µg |
| Related Products | HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies |
| Shipping | This product is shipped in non-frozen liquid form in ambient conditions |
| Storage | Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles |
| References | Jung HJ, Park Y, Hahm KS, Lee DG.(2006) Biological activity of Tat (47-58) peptide on human pathogenic fungi. Biochem Biophys Res Commun. 345: 222-8. |
| Limitations | This product is for research use only TECHNICAL ASSISTANCE Please refer any technical questions to technical.support@icosagen.com |
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€299 / 100µg
Figure
1: Western Blot testing of HIV-1 recombinant Tat
protein. using HIV-1 Tat antibody (clone N3). Lane 1. Recombinant HIV-1
Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size
marker
Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone N3). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA