Antibodies

Mouse monoclonal antibody to HIV-1 Tat

A2-823-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Tat
Immunogen HIV-1 Tat
Immunogen Description Recombinant Tat protein of HIV-1 BH10 (purified from E. coli)
Clonality Mouse monoclonal
Clone 15
Class IgG1
Reactivity Reacts HIV-1 Tat of BH10, IIIB strains. HIV-1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). Mapped to amino acids 2-9 (EPVDPRLE ­- B subtype a.a. consensus)
Application ELISA, WB, ChIP
Protocol ELISA 0.2 to 1 µg/ml; WB 2 µg/ml

Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at –20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
References

Campioni, D., Corallini, A., Zauli, G., Possati, L., Altavilla, G. and Barbanti-Brodano, G (1995). HIV type I extracellular Tat protein stimulates growth and protects cells of BK virus/tat transgenic mice from apoptosis. AIDS Res Hum Retroviruses 11, 1039-1048. PMID: 8554901

Huang L, Bosch I, Hofmann W, Sodroski J, Pardee AB. (1998). Tat protein induces human immunodeficiency virus type 1 (HIV-1) coreceptors and promotes infection with both macrophage-tropic and T-lymphotropic HIV-1 strains. J Virol. 72(11):8952-60. PMID: 9765440

Rohr O, C Schwartz, C Hery, D Aunis, M Tardieu and E Schaeffer (2000). The nuclear receptor COUP-TF interacts with HIV-1 Tat and stimulates viral replication in human microglial cells. Journal of Biological Chemistry. 28;275(4): 2654-60. PMID: 10644726

Arp J, Rieder MJ, Urquhart B, Freeman D, Tucker MJ, Krizova A, Lehmann D, Dekaban GA (2005). Hypersensitivity of HIV-1 infected cells to reactive sulfonamide metabolites correlated to expression of the HIV-1 viral protein tat. J. Pharmcol Exp Ther 314:1218-25. PMID: 15933158

Karasev AV, Foulke S, Wellens C, Rich A, Shon KJ, Zwierzynski I, Hone D, Koprowski H, Reitz M. (2005). Plant based HIV-1 vaccine candidate: Tat protein produced in spinach. Vaccine. 7;23(15):1875-80. PMID: 15734059

Abbott KL, Archambault J, Xiao H, Nguyen BD, Roeder RG, Greenblatt J, Omichinski JG, Legault P. (2005) Interactions of the HIV-1 Tat and RAP74 Proteins with the RNA Polymerase II CTD Phosphatase FCP1. Biochemistry 44, 2716-2731. PMID: 15723517

Rolland M, Jensen MA, Nickle DC,Yan J, Learn GH,1 Heath L, Weiner D, Mullins JI.(2007) Reconstruction and Function of Ancestral Center-of-Tree Human Immunodeficiency Virus Type 1 Proteins. Journal of Virology,  Vol. 81, No. 16
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

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€299 / 100µg













Figure 1.  Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone 15). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml.  Lane 2. Protein size marker



Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone 15). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA