Antibodies

Mouse monoclonal antibody to HIV-1 Tat

A2-822-100

Datasheet as
Name Mouse monoclonal antibody to HIV-1 Tat
Immunogen HIV-1 Tat
Immunogen Description Recombinant Tat protein of HIV-1 BH10 (purified from E. coli)
Clonality Mouse monoclonal
Clone 7
Class IgG1
Reactivity Reacts in ELISA, WB with HIV-1 Tat of BH10, IIIB strains. This antibody reacts with the rTat consensus sequences of HIV-1 subtypes A (PNLEPWH), B (PRLEPWK) and C (PNLEPWN). Mapped to amino acids 6-12 (PRLEPWK - B subtype a.a. consensus)
Application ELISA, WB, ChIP
Protocol ELISA 0.05 to 0.1 µg/ml; WB 1 to 2 µg/ml. 
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
Purification Protein G purification
Buffer PBS pH 7.4, with 0.1% sodium azide
Unit Size 100 µg
Related Products HIV-1 Tat monoclonal antibodies. For more information visit www.icosagen.com/antibodies
Shipping This product is shipped in non-frozen liquid form in ambient conditions
Storage Store at -20…-70°C upon receipt. Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles
Background -
References Reid W., Sadowska M., Denaro, F. Rao S., Foulke J., Hayes N., Jones 0., Davis H., Sill A., O'Driscoll P., Huso, D., Fouts T., Lewis, G., Kames-Lowis R., Wei C., Ray P, Gallo, R., Reits, M., and Bryant J. (2001) An HIV -1 Transgenic Rat that Develops HIV -1 related Pathology and Immunological Dysfunction. Proceedings of the National Academy of Science, 98 (16): 9271-9276.
Limitations

This product is for research use only

TECHNICAL ASSISTANCE

Please refer any technical questions to technical.support@icosagen.com

Buy now
€299 / 100µg













Figure 1. Western Blot testing of HIV-1 recombinant Tat protein. using HIV-1 Tat antibody (clone 7). Lane 1. Recombinant HIV-1 Tat (B subtype), 100 ng. Antibody concentration 2 µg/ml. Lane 2. Protein size marker


Figure 2. Matrix-ChIP analysis of HIV-1 Tat antibody (clone 7). Human 5A8 J-lat T lymphocytes culture was latently infected with HIV and treated with 10 ug/mL PHA